NAT1 alleles in non-human eukaryotes

 

 

Updated May 2024

 

 

NAT1 allele (haplotype)(a)

Retired & discontinued NAT allele(a)

Nucleotide change(s)(b)

Amino acid change(s)(b)

Organism

Literature

(RABIT)NAT1*1

NAT1*1

Reference

Reference

Rabbit

(Oryctolagus cuniculus)

2

(RABIT)NAT1*2

NAT1*2

None in coding region

None

Rabbit

(Oryctolagus cuniculus)

3

(MOUSE)Nat1*1

NAT1*6

Reference

Reference

Mouse

(Mus musculus)

4-9(c)

(MUSSP)Nat1*2

Nat1*30

c.642A>G

c.684G>A

c.695A>G

c.699T>C

c.795T>C

p.Ala214=

p.Ser228=

p.His232Arg

p.Cys233=

p.Asp265=

Western wild mouse

(Mus spretus)

8(c)

(MESAU)Nat1*1

NAT1*8

Reference

Reference

Syrian hamster

(Mesocricetus auratus)

10-12

(MESAU)Nat1*2

NAT1*9

c.60T>C

p.Asp20=

Syrian hamster

(Mesocricetus auratus)

11

(MACMU)NAT1*1

 

 

Reference

Reference

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*2

 

c.244A>G

c.321C>T

c.540C>T

p.Met82Val

p.His107=

p.Leu180=

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*3

 

c.321C>T

p.His107=

 

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*4

 

c.152G>C

c.321C>T

p.Gly51Ala

p.His107=

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*5

 

c.321C>T

c.540C>T

p.His107=

p.Leu180=

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*6

 

c.321C>T

c.523T>C

c.540C>T

p.His107=

p.Phe175Leu

p.Leu180=

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*7

 

c.321C>T

c.560G>A

p.His107=

p.Arg187Gln

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*8

 

c.15A>G

p.Ala5=

 

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*9

 

c.321C>T

c.493A>C

p.His107=

p.Arg165=

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*10

 

c.267G>C

c.321C>T

c.343G>T

c.463G>C

p.Leu89Phe

p.His107=

p.Asp115Tyr

p.Glu155Gln

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*11

 

c.321C>T

c.343G>T

p.His107=

p.Asp115Tyr

Rhesus macaque

(Macaca mulatta)

13

(MACMU)NAT1*12

 

c.177C>T

c.321C>T

p.Val59=

p.His107=

Rhesus macaque

(Macaca mulatta)

13

 

 

Footnotes

(a)         The gene symbols are assigned according to the current guidelines of the NAT Gene Nomenclature Committee. Previous NAT alleles that did not follow the current convention have been retired and discontinued [1]. The alleles of rodents are written as Nat (first letter uppercase and second and third letter lowercase). The alleles of all other species are all uppercase (e.g. NAT).

(b)         The position of SNPs in each ORF is determined relative to the A of the ATG translation initiation codon, which is always considered as number 1. The first methionine of each NAT protein is amino acid number 1, with polymorphic amino acid positions determined accordingly. The reference allele of each NAT1 gene (usually the wild type allele or the first allele identified in a specific organism) is assigned symbol NAT1*1.

(c)         Studied western European house mouse (Mus musculus) laboratory strains: i) inbred C57Bl/6J [4,5], Balb/c [6], C3H/HeJ [7], 129/Ola [8], CBA [8], A/J [4,5] and A/HeJ [7], ii) outbred CD1 [9], PO [8] and TO [8]. Also, the wild-derived inbred strains Mus spretus (western wild mouse) and Mus musculus castaneus (southestern Asian house mouse) [8]. All strains, except Mus spretus, carry the wild type allele (MOUSE)Nat1*1 at the Nat1 locus.

 

For new submissions or enquiries, please contact Dr. Sotiria Boukouvala (sboukouv@mbg.duth.gr).

 

 

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Literature

[1]     Hein, D.W.; Boukouvala, S.; Grant, D.M.; Minchin, R.F. and Sim, E. (2008) Changes in consensus arylamine N-acetyltransferase gene nomenclature. Pharmacogenet. Genomics 18(4), 367-368.

[2]     Blum, M.; Heim, M. and Meyer, U.A. (1990) Nucleotide sequence of rabbit NAT1 encoding monomorphic arylamine N-acetyltransferase. Nucl. Acids Res. 18(17), 5287.

[3]     Sasaki, Y.; Ohsako, S. and Deguchi, T. (1991) Molecular and genetic analyses of arylamine N-acetyltransferase polymorphism of rabbit liver. J. Biol. Chem. 266(20), 13243-13250.

[4]     Martell, K.J.; Vatsis, K.P. and Weber, W.W. (1991) Molecular genetic basis of rapid and slow acetylation in mice. Mol. Pharmacol. 40(2), 218-227.

[5]     Martell, K.J.; Levy, G.N. and Weber, W.W. (1992) Cloned mouse N-acetyltransferases: Enzymatic properties of expressed Nat1 and Nat2 gene products. Mol. Pharmacol. 42(2), 265-272.

[6]     Kelly, S.L. and Sim, E. (1994) Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro. Biochem. J. 302(2), 347-353.

[7]     Fretland, A.J.; Doll, M.A.; Gray, K.; Feng, Y. and Hein, D.W. (1997) Cloning, expression and recombinant expression of NAT1, NAT2, and NAT3 derived from C3H/HeJ (rapid) and A/HeJ (slow) acetylator inbred mouse: Functional characterization of the activation and deactivation of aromatic amine carcinogens. Toxicol. Appl. Pharmacol. 142(2), 360-366.

[8]     Boukouvala, S.; Price, N. and Sim, E. (2002) Identification and functional characterization of novel polymorphisms associated with the genes for arylamine N-acetyltransferases in mice. Pharmacogenetics 12(5), 385-394.

[9]     Estrada, L.; Kanelakis, K.C.; Levy, G.N. and Weber, W.W. (2000) Tissue- and gender-specific expression of N-acetyltransferase 2 (NAT2*) during development of the outbred mouse strain CD-1. Drug Metab. Dispos. 28(2), 139-146.

[10]    Abu-Zeid, M.; Nagata, K.; Miyata, M.; Ozawa, S.; Fukuhara, M.; Yamazoe, Y. and Kato, R. (1991) An arylamine acetyltransferase (AT-1) from Syrian golden hamster liver: Cloning, complete nucleotide sequence and expression in mammalian cells. Mol. Carcinogen. 4(1), 81-88.

[11]    Ferguson, R.J., Doll, M.A., Rustan, T.D., Baumstark, B.R. and Hein, D.W. (1994) Syrian hamster monomorphic N-acetyltransferase (NAT1) alleles: Amplification, cloning, sequencing, and expression in E. coli. Pharmacogenetics 4(2), 82-90.

[12]    Land, S.J.; Jones, R.E. and King, C.M. (1994) Biochemical and genetic analysis of two acetyltransferases from hamster tissues that can metabolise aromatic amine derivatives. Carcinogenesis 15(8), 1585-1595.

[13]    Boukouvala, S.; Chasapopoulou, Z.; Giannouri, D.; Kontomina, E.; Marinakis, N.; Rizou. S.V.; Stefani, I.; Tsirka, T.; Veyssière, C.; Zaliou, S.; Sabbagh, A.; Crouau-Roy, B. and Fakis, G. (2019) Population variability of rhesus macaque (Macaca mulatta) NAT1 gene for arylamine N-acetyltransferase 1: Functional effects and comparison with human. Sci. Rep. 9, 10937

 

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